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The Synthesis of Magnetic Lysozyme‐Imprinted Polymers by Means of Distillation–Precipitation Polymerization for Selective Protein Enrichment
Author(s) -
Cao Jiali,
Zhang Xihao,
He Xiwen,
Chen Langxing,
Zhang Yukui
Publication year - 2014
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201300937
Subject(s) - precipitation polymerization , molecularly imprinted polymer , fourier transform infrared spectroscopy , thermogravimetric analysis , materials science , polymerization , magnetic nanoparticles , adsorption , chemical engineering , nanoparticle , copolymer , polymer , scanning electron microscope , chemistry , nanotechnology , radical polymerization , organic chemistry , selectivity , composite material , catalysis , engineering
A protein imprinting approach for the synthesis of core–shell structure nanoparticles with a magnetic core and molecularly imprinted polymer (MIP) shell was developed using a simple distillation–precipitation polymerization method. In this work, Fe 3 O 4 magnetic nanoparticles were first synthesized through a solvothermal method and then were conveniently surface‐modified with 3‐(methacryloyloxy)propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high‐density MIP shell was coated onto the surface of the magnetic nanoparticles by the copolymerization of functional monomer acrylamide (AAm), cross‐linking agent N , N ′‐methylenebisacrylamide (MBA), the initiator azodiisobutyronitrile (AIBN), and protein in acetonitrile heated at reflux. The morphology, adsorption, and recognition properties of the magnetic molecularly imprinted nanoparticles were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and rebinding experiments. The resulting MIP showed a high adsorption capacity (104.8 mg g −1 ) and specific recognition (imprinting factor=7.6) to lysozyme (Lyz). The as‐prepared Fe 3 O 4 @Lyz‐MIP nanoparticles with a mean diameter of 320 nm were coated with an MIP shell that was 20 nm thick, which enabled Fe 3 O 4 @Lyz‐MIP to easily reach adsorption equilibrium. The high magnetization saturation (40.35 emu g −1 ) endows the materials with the convenience of magnetic separation under an external magnetic field and allows them to be subsequently reused. Furthermore, Fe 3 O 4 @Lyz‐MIP could selectively extract a target protein from real egg‐white samples under an external magnetic field.