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Increased Efficacies in Magnetofection and Gene Delivery to Hepatocellular Carcinoma Cells with Ternary Organic–Inorganic Hybrid Nanocomposites
Author(s) -
Leung Ken ChamFai,
Chak ChunPong,
Lee SiuFung,
Lai Josie M. Y.,
Zhu XiaoMing,
Wang YiXiang J.,
Sham Kathy W. Y.,
Wong ChiHin,
Cheng Christopher H. K.
Publication year - 2013
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201300202
Subject(s) - polyethylenimine , transfection , nanocomposite , gene delivery , ternary operation , materials science , plasmid , chemistry , microbiology and biotechnology , dna , nanotechnology , gene , biology , biochemistry , computer science , programming language
A magnetic personality : Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic–inorganic hybrid nanocomposites that contain deferoxamine‐coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.