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Turn‐On Fluorescence Detection of Apoptotic Cells Using a Zinc(II)‐Dipicolylamine‐Functionalized Poly(diacetylene) Liposome
Author(s) -
Cho YongSuk,
Kim Kyung Mi,
Lee Duhwan,
Kim Won Jong,
Ahn Kyo Han
Publication year - 2013
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201201139
Subject(s) - phosphatidylserine , liposome , diacetylene , fluorescence , chemistry , confocal , confocal microscopy , fluorescence microscope , biophysics , biochemistry , microbiology and biotechnology , organic chemistry , biology , phospholipid , membrane , optics , physics , polymerization , polymer
A liposome‐based fluorescence sensing system for apoptotic cells has been developed from stimuli‐responsive poly(diacetylene)‐liposomes for the first time. The combination of the liposome components, a phosphatidylserine‐binding Zn II ‐dipicolylamine component and an alcohol‐terminated component in the ratio of 2:1, has led to an efficient detection system for apoptotic cells, as demonstrated by confocal fluorescence microscopy and FACS analysis. The liposome shows a color change from blue to reddish purple and emits fluorescence in the turn‐on mode upon interaction with phosphatidylserine. The present system thus avoids the washing steps required for “always‐on”‐type sensing systems.