Real‐Time Fluorescence Turn‐On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe | Zendy

Chen Jian | Zendy; Jiao Huping | Zendy; Li Wenying | Zendy; Liao Dongli | Zendy; Zhou Huipeng | Zendy; Yu Cong | Zendy

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Real‐Time Fluorescence Turn‐On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe

Author(s) -

Chen Jian,

Jiao Huping,

Li Wenying,

Liao Dongli,

Zhou Huipeng,

Yu Cong

Publication year - 2013

Publication title -

chemistry – an asian journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.18

H-Index - 106

eISSN - 1861-471X

pISSN - 1861-4728

DOI - 10.1002/asia.201200880

Subject(s) - fluorescence , perylene , alkaline phosphatase , chemistry , monomer , molecular probe , photochemistry , biophysics , biochemistry , enzyme , biology , organic chemistry , optics , polymer , dna , physics

A tetracationic perylene probe (probe 2 ) was designed and synthesized. Probe 1 was used for the real‐time fluorescence turn‐on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.

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