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Preparation of High‐Performance Water‐Soluble Quantum Dots for Biorecognition through Fluorescence Resonance Energy Transfer
Author(s) -
Huang ChinPing,
Chao ChengFu,
Shen MoYuan,
Chen TengMing,
Li YawKuen
Publication year - 2012
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201200526
Subject(s) - quantum dot , quantum yield , fluorescence , förster resonance energy transfer , photoluminescence , adsorption , chemistry , nanotechnology , energy transfer , luminescence , chemical engineering , photochemistry , materials science , optoelectronics , organic chemistry , chemical physics , physics , quantum mechanics , engineering
An improved method for the synthesis of high‐performance and water‐soluble quantum dots (QDs) involving the encapsulation of mercaptosuccinic acid coated QDs (MSA‐QDs) with poly(diallyldimethylammonium chloride) (PDDA) followed by their direct photoactivation with fluorescent radiation near 295 K to yield PDDA‐coated QDs (PDDA‐QDs) has been demonstrated. The quantum yield (QY) of the PDDA‐QDs was significantly improved from 0.6 (QY of MSA‐QDs) to 48 %. By using this synthetic strategy, highly photoluminescent PDDA‐QDs of varied size were readily prepared. The surface properties of PDDA‐QDs and MSA‐QDs were extensively characterized. The highly luminescent and positively charged PDDA‐QDs serve as a useful and convenient tool for protein adsorption. With a Δ 5 ‐3‐ketosteroid isomerase adsorbed PDDA‐QD complex, the biorecognition of steroids was demonstrated through the application of fluorescent resonance energy transfer.