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Stable Sugar‐Chain‐Immobilized Fluorescent Nanoparticles for Probing Lectin and Cells
Author(s) -
Shinchi Hiroyuki,
Wakao Masahiro,
Nakagawa Sho,
Mochizuki Eiko,
Kuwabata Susumu,
Suda Yasuo
Publication year - 2012
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201200362
Subject(s) - lectin , concanavalin a , sugar , chemistry , fluorescence , biophysics , galactose , agglutinin , in vivo , biochemistry , nanoparticle , ligand (biochemistry) , in vitro , nanotechnology , materials science , biology , receptor , physics , microbiology and biotechnology , quantum mechanics
Abstract Sugar chains are important molecules in cellular recognition and signaling, and quantum dots (QDs) are a very powerful tool for in vitro and in vivo imaging. Herein, we report the preparation of stable sugar‐chain‐immobilized fluorescent nanoparticles (SFNPs) and their application to the analysis of sugar‐chain–protein interactions and cellular imaging. SFNPs were easily prepared by mixing CdTe/CdS core/shell QDs with our previously developed sugar‐chain–ligand conjugates. The obtained SFNPs were very stable and could be stored for several months. In the binding analysis, β‐galactose‐ and α‐glucose‐immobilized SFNPs were specifically interacted with Ricinus communis agglutinin I and concanavalin A, respectively, and made into aggregates. The binding interaction was detected visually, fluorescently, or both. In the experiment for cellular imaging, it was found that SFNPs were predominantly taken up by human hepatocyto carcinoma cells (HepG2), suggesting the possible usage of our designed SFNPs for various biochemical analyses of sugar chains.