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Single‐Step Turn‐On Homogeneous Fluorescent Immunosensor for Rapid, Sensitive, and Selective Detection of Proteins
Author(s) -
Wang Dan,
Zhu Xiaocui,
Zhao Dongqin,
Cai Yingxiao,
Zhao Meiping
Publication year - 2012
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201200086
Subject(s) - homogeneous , fluorescence , covalent bond , chemistry , colloidal gold , turn (biochemistry) , chromatography , nanotechnology , nanoparticle , materials science , biochemistry , optics , physics , organic chemistry , thermodynamics
Quick & easy : A simple and positive‐readout fluorescent immunosensor was developed based on a quencher composed of a 3 nm gold nanoparticle (GNP) covalently linked to a Fab fragment, which proved to be stable and specific. The method allows for direct and sensitive detection of target antigens in homogeneous solutions in one step without any separation steps. It has been successfully applied to rapidly quantify the amount of secretory immunoglobulin A (sIgA) in human saliva samples.