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Cover Picture: Cassia obtusifolia MetE as a Cytosolic Target for Potassium Isolespedezate, a Leaf‐Opening Factor of Cassia plants: Target Exploration by a Compact Molecular‐Probe Strategy (Chem. Asian J. 12/2011)
Author(s) -
Ueda Minoru,
Manabe Yoshiyuki,
Otsuka Yuki,
Kanzawa Nobuyuki
Publication year - 2011
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201190050
Subject(s) - cassia , ligand (biochemistry) , chemistry , molecule , conjugated system , combinatorial chemistry , organic chemistry , receptor , biochemistry , polymer , medicine , alternative medicine , pathology , traditional chinese medicine
Affinity chromatography (AC) using ligand‐immobilized bead technology is generally the first choice for target exploration. However, a moderately bioactive ligand, such as endogenous bioactive metabolites, is expected to cause difficulties in AC‐based target detection because AC is highly dependent to extremely high affinity between ligand and target. As described in their Full Paper on 3286 ff., M. Ueda et al. report that a compact molecular probe (CMP) strategy has proven to be effective for detection of a moderate‐affinity target for which AC cannot be applied successfully. We applied this CMP strategy for the target identification of potassium isolespedezate that showed moderate leaf‐opening activity (10 −5 –10 −6 M ). By using this CMP approach, the cytosolic target protein, Cassia obtusifolia MetE, was detected and isolated as a single band.