Premium
Synthesis of 2′‐Deoxy‐5‐(methylselenyl)cytidine and Se‐DNAs for Structural and Functional Studies
Author(s) -
Zhang Wen,
Sheng Jia,
Hassan Abdalla E.,
Huang Zhen
Publication year - 2012
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201100898
Subject(s) - cytidine , phosphoramidite , deoxycytidine , chemistry , methylation , dna , dna synthesis , stereochemistry , combinatorial chemistry , biochemistry , oligonucleotide , biology , genetics , enzyme , chemotherapy , gemcitabine
It's not in your DNA : We report here the synthesis of a novel 5‐methyl deoxycytidine analog and its phosphoramidite modified with selenium at the C‐5 position ( Se C). Incorporation of Se C into DNA does not cause structural perturbations, as shown by X‐ray crystallography and in agreement with UV melting data. The Se modification provides a useful strategy for biochemical and structural investigations on 5‐methylation of cytidine.