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Fluorogenic Protein Labeling through Photoinduced Electron Transfer‐Based BL‐Tag Technology
Author(s) -
Sadhu Kalyan K.,
Mizukami Shin,
Lanam Carolyn R.,
Kikuchi Kazuya
Publication year - 2012
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201100647
Subject(s) - photoinduced electron transfer , fluorescence , quenching (fluorescence) , moiety , linker , chemistry , electron transfer , kinetics , photochemistry , fluorescent protein , mutant , biophysics , green fluorescent protein , biochemistry , stereochemistry , biology , physics , quantum mechanics , computer science , gene , operating system
Keeping quenchers on a short leash: Shortening of the linker chain length combined with modification of the quencher moiety was found to improve fluorogenic probes useful for protein labeling by the mutant β‐lactamase‐tag (BL‐tag) technology. The most effective probe, CC3 DNB, displays fluorescence quenching by photoinduced electron transfer (PET) and results in fluorescent protein labeling with fast kinetics.