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An Anticoagulant Activity System Using Nanoengineered Autofluorescent Heparin Nanotubes
Author(s) -
Cui Wei,
Cui Yue,
Zhu Pengli,
Zhao Jie,
Su Ying,
Yang Yang,
Li Junbai
Publication year - 2012
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201100425
Subject(s) - autofluorescence , materials science , scanning electron microscope , transmission electron microscopy , chitosan , heparin , confocal microscopy , nanomaterials , confocal , nanotechnology , anticoagulant , fluorescence , chemical engineering , covalent bond , chemistry , organic chemistry , biochemistry , optics , composite material , medicine , physics , engineering
Heparin (HEP) and periodate‐oxidized heparin (O‐HEP) nanotubes were prepared by combining the template method with a layer‐by‐layer (LbL) technique. The tubular structure was obtained and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal laser scanning microscopy (CLSM). O‐HEP is one of the HEP derivatives that contains anticoagulant activity and preserves its ability for other effects. Chitosan (CHI) and O‐HEP have been used to fabricate nanotubes by covalent cross‐linking Schiff base reactions . It is demonstrated that the obtained nanotubes have the significant feature of autofluorescence without the addition of any fluorescent dyes and they retain their anticoagulation activity. Compared with O‐HEP/CHI nanotubes, HEP/CHI nanotubes show high anticoagulation activity and do not have autofluorescence. Furthermore, this method could be extended to other copolysaccharide derivatives for the preparation of autofluorescent nanomaterials.