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Nanoparticle‐based Indicator‐Displacement Assay for Pyrophosphate
Author(s) -
Oh Dong Ju,
Kim Kyung Mi,
Ahn Kyo Han
Publication year - 2011
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201100149
Subject(s) - pyrophosphate , nanoparticle , receptor , chemistry , phosphate , molecular recognition , displacement (psychology) , derivative (finance) , absorption (acoustics) , size exclusion chromatography , molecule , materials science , nanotechnology , enzyme , biochemistry , organic chemistry , psychology , financial economics , economics , psychotherapist , composite material
Abstract Silica nanoparticles functionalized with a Zn II ‐dipicolylamine derivative are used as the receptor component of a sensing ensemble for an indicator‐displacement assay. The nano‐ensemble system, constructed with pyrocatechol violet as an indicator, selectively senses pyrophosphate over other anions including hydrogen phosphate, thus showing a visible blue‐to‐yellow color change and the corresponding absorption spectral changes in a buffer solution of pH 7. A distinct recognition behavior—the nano‐ensemble does not sense hydrogen phosphate that was sensed by the traditional ensemble with the same molecular receptor—is explained by an incomplete‐displacement model in the case of the integrated receptor system. The present work demonstrates that the nanoparticle‐based competition assay is as effective as the traditional approach with molecular receptors. Furthermore, such an integrated receptor system can provide distinct recognition behavior from its molecular receptor.