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Facile One‐Step Catalytic Grafting of N ‐Hydroxysuccinimidyl‐Ester‐Functionalized Methallylsilane onto Silica for Enzyme Immobilization
Author(s) -
Jung UnYoung,
Park JungWoo,
Han EunHee,
Kang SinGun,
Lee Sera,
Jun ChulHo
Publication year - 2011
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201000713
Subject(s) - grafting , reagent , catalysis , mesoporous silica , immobilized enzyme , covalent bond , chemistry , polymer chemistry , one step , enzyme , mesoporous material , chemical engineering , combinatorial chemistry , materials science , organic chemistry , polymer , engineering
A one‐step grafting method of NHS‐ester group is described for the immobilization of enzymes onto a silica surface at room temperature. Pure NHS‐ester functionalized methallylsilane was prepared as a grafting reagent and showed remarkable grafting efficiency with acid catalysts at room temperature. As a result, mesoporous silica balls bearing a covalently immobilized enzyme could be obtained with a high loading rate compared to conventional multistep grafting methods. This one‐step procedure offers the advantages of precise measurement of the high loading efficiency of the NHS‐ester group, and the exclusive formation of only the NHS‐ester group, which reduces the presence of unreacted functionalities.