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Peering into Biological Nanopore: A Practical Technology to Single‐Molecule Analysis
Author(s) -
Wang HaiYan,
Ying YiLun,
Li Yang,
Long YiTao
Publication year - 2010
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201000279
Subject(s) - nanopore , peering , nanopore sequencing , nanotechnology , molecule , computational biology , materials science , computer science , chemistry , dna , dna sequencing , biology , biochemistry , the internet , organic chemistry , world wide web
As a unique technique at the singe‐molecule level to explore the distribution and temporal order of events, nanopore technology has attracted increasing attention. In comparison to the previous applications in DNA sequencing, this Focus Review highlights the technical details of biological nanopores, especially α‐hemolysin, in the analysis of peptides and proteins. The instrument configurations, experimental interferences, and data analysis including the conformation of peptides and proteins and their interactions for single‐molecule detection are discussed.