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N 6 ‐(2‐( R )‐Propargylglycyl)lysine as a Clickable Pyrrolysine Mimic
Author(s) -
Li Xin,
Fekner Tomasz,
Chan Michael K.
Publication year - 2010
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201000205
Subject(s) - click chemistry , dipeptide , chemistry , azide , calmodulin , lysine , fluorescence , coumarin , combinatorial chemistry , yield (engineering) , bioorthogonal chemistry , copycat , biochemistry , amino acid , computer science , organic chemistry , enzyme , physics , quantum mechanics , materials science , artificial intelligence , metallurgy
Clickable copycat! Readily available dipeptide D ‐Pra‐ ε ‐Lys is identified using a modified fluorescence protein assay as a highly efficient clickable pyrrolysine mimic. It is shown to incorporate into calmodulin in high yield to provide a handle for labeling with an azide‐containing coumarin.