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Generation of Patterned Neuronal Networks on Cell‐Repellant Poly(oligo(ethylene glycol) Methacrylate) Films
Author(s) -
Kang Kyungtae,
Kang Gyumin,
Lee Bong Soo,
Choi Insung S.,
Nam Yoonkey
Publication year - 2010
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.200900761
Subject(s) - ethylene glycol , methacrylate , materials science , polymer chemistry , chemical engineering , chemistry , polymer , polymerization , organic chemistry , composite material , engineering
The utilization of non‐biofouling poly(oligo(ethylene glycol) methacrylate) ( p OEGMA) films as a background material for the generation of neuronal patterns is reported here. Our previously reported method, which was surface‐initiated, atom transfer radical polymerization of OEGMA, and subsequent activation of terminal hydroxyl groups of p OEGMA with disuccinimidyl carbonate, was employed for the generation of activated p OEGMA films on glass. Poly‐ L ‐lysine was then microcontact‐printed onto the activated polymer films , followed by backfilling with poly(ethylene glycol) moieties. E18 hippocampal neurons were cultured on the chemically patterned substrate, and the resulting neuronal networks were analyzed by phase‐contrast microscopy and whole‐cell patch clamp method. The results indicated that the p OEGMA films played an important role in the generation of good‐quality neuronal patterns for up to two weeks without any negative effects to neurons.