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Electrografted Poly( N ‐mercaptoethyl acrylamide) and Au Nanoparticles‐Based Organic/Inorganic Film: A Platform for the High‐Performance Electrochemical Biosensors
Author(s) -
Li Limiao,
Xu Shoujiang,
Du Zhifeng,
Gao Yanfang,
Li Jinghong,
Wang Taihong
Publication year - 2010
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.200900490
Subject(s) - biosensor , acrylamide , horseradish peroxidase , nanoparticle , electrode , electrochemistry , redox , materials science , biomolecule , nanotechnology , chemical engineering , chemistry , inorganic chemistry , polymer , organic chemistry , enzyme , copolymer , engineering , composite material
In this study, we describe the use of the combination of eletrografting poly( N ‐mercaptoethyl acrylamide) and Au nanoparticles in the construction of high‐performance biosensors. The poly( N ‐mercaptoethyl acrylamide) was electrografted onto the glassy carbon electrode surface, which provided a strongly adhering primer film for the stable attachment of Au nanoparticles and horseradish peroxidase (HRP) enzymes. The performances of the biosensors based on the HRP immobilized in the Au/poly( N ‐mercaptoethyl acrylamide) composite film were investigated. A couple of redox peaks were obtained, indicating that the Au nanoparticles could facilitate the direct‐electron transfer between HRP and the underlying electrode. The biosensor showed an excellent electrocatalytic activity toward the reduction of hydrogen oxide and long‐term stability, owing to the stable electrografted film and biocompatible Au nanoparticles. Our results demonstrate that the combination of electrografting and Au nanoparticles provides a promising platform for the immobilization of biomolecules and analysis of redox enzymes for their sensing applications.