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Magnetic Silica‐Coated Sub‐Microspheres with Immobilized Metal Ions for the Selective Removal of Bovine Hemoglobin from Bovine Blood
Author(s) -
Zhang Min,
Cheng Dan,
He Xiwen,
Chen Langxing,
Zhang Yukui
Publication year - 2010
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.200900463
Subject(s) - superparamagnetism , adsorption , magnetite , materials science , scanning electron microscope , metal ions in aqueous solution , transmission electron microscopy , magnetic nanoparticles , protein adsorption , nuclear chemistry , metal , chemical engineering , chromatography , analytical chemistry (journal) , chemistry , magnetization , nanotechnology , nanoparticle , composite material , metallurgy , organic chemistry , physics , engineering , quantum mechanics , magnetic field
Abstract Magnetic silica‐coated magnetite (Fe 3 O 4 ) sub‐microspheres with immobilized metal‐affinity ligands are prepared for protein adsorption. First, magnetite sub‐microspheres were synthesized by a hydrothermal method. Then silica was coated on the surface of Fe 3 O 4 particles using a sol–gel method to obtain magnetic silica sub‐microspheres with core‐shell morphology. Next, the trichloro(4‐chloromethylphenyl) silane was immobilized on them, reacted with iminodiacetic acid (IDA), and charged with Cu 2+ . The obtained magnetic silica sub‐microspheres with immobilized Cu 2+ were applied for the absorption of bovine hemoglobin (BHb) and the removal of BHb from bovine blood. The size, morphology, and magnetic properties of the resulting magnetic micro(nano) spheres were investigated by using scanning microscopy (SEM), transmission electron microscopy (TEM), X‐ray diffraction (XRD), and a vibrating sample magnetometer (VSM). The measurements showed that the magnetic sub‐microspheres are spherical in shape, very uniform in size with a core‐shell, and are almost superparamagnetic. The saturation magnetization of silica‐coated magnetite (Fe 3 O 4 ) sub‐microspheres reached about 33 emu g −1 . Protein adsorption results showed that the sub‐microspheres had a high adsorption capacity for BHb (418.6 mg g −1 ), low nonspecific adsorption, and good removal of BHb from bovine blood. This opens a novel route for future applications in removing abundant proteins in proteomic analysis.