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Photoassisted Synthesis of Luminescent Mannose–Au Nanodots for the Detection of Thyroglobulin in Serum
Author(s) -
Huang ChihChing,
Hung YuLun,
Shiang YenChun,
Lin TaiYuan,
Lin YuShen,
Chen ChaoTsen,
Chang HuanTsung
Publication year - 2010
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.200900346
Subject(s) - luminescence , quantum yield , concanavalin a , quenching (fluorescence) , photochemistry , phosphorescence , nanodot , chemistry , mannose , nanoparticle , luminescent measurements , mannose receptor , materials science , nanotechnology , fluorescence , optoelectronics , organic chemistry , biochemistry , in vitro , optics , physics , macrophage
We have employed mannose‐modified gold nanodots (Man–Au NDs) as a luminescence sensor for the detection of the thyroid‐cancer marker thyroglobulin (Tg) in homogeneous solutions. The luminescent Man–Au NDs are prepared through the reaction of 2.9 nm‐diameter gold nanoparticles (Au NPs) with 11‐mercapto‐3,6,9‐trioxaundecyl‐α‐ D ‐mannopyranoside (Man‐RSH) under the irradiation of a light‐emitting diode (LED). We have found that the irradiation enhances the quantum yield (∼11 %), alters the emission wavelength and lifetimes, and shortens the preparation time. A luminescence assay has been developed for Tg based on the competition between Tg and Man–Au NDs for the interaction with the concanavalin A (Con A). Because luminescence quenching of the Man–Au NDs by Con A is inhibited by Tg selectivity, we have obtained a highly sensitive and selective assay for Tg.