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Cover Picture: Hybridization‐Sensitive On–Off DNA Probe: Application of the Exciton Coupling Effect to Effective Fluorescence Quenching (Chem. Asian J. 6/2008)
Author(s) -
Ikeda Shuji,
Okamoto Akimitsu
Publication year - 2008
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.200890018
Subject(s) - fluorescence , exciton , quenching (fluorescence) , absorption (acoustics) , chemistry , photochemistry , coupling (piping) , analytical chemistry (journal) , materials science , optoelectronics , physics , optics , condensed matter physics , metallurgy , chromatography
A doubly fluorescence‐labeled nucleoside is designed that possesses an absorption band at approximately 480 nm observed when the probe is in a single‐stranded state, and an absorption band at approximately 510 nm when the probe is hybridized with the complementary strand. This clear change in fluorescence intensity which is dependent on hybridization is useful for visible gene analysis. The present approach, developed by S. Ikeda and A. Okamoto, includes many advantages that are quite different from conventional assays. For details, turn to their Full Paper on page 958 ff.