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Increased Circulating Follicular Treg Cells Are Associated With Lower Levels of Autoantibodies in Patients With Rheumatoid Arthritis in Stable Remission
Author(s) -
Liu Chen,
Wang Dongwei,
Lu Songsong,
Xu Qinzhu,
Zhao Lei,
Zhao Jingzhong,
Song Ying,
Wang Hui
Publication year - 2018
Publication title -
arthritis and rheumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.106
H-Index - 314
eISSN - 2326-5205
pISSN - 2326-5191
DOI - 10.1002/art.40430
Subject(s) - rheumatoid arthritis , immunology , autoantibody , foxp3 , flow cytometry , medicine , rheumatoid factor , follicular phase , pathogenesis , antibody , immune system
Objective To examine the expression and changes in function of circulating CD 4+ CXCR 5+FoxP3+ follicular Treg (Tfr) cells in patients with active rheumatoid arthritis ( RA ) and in patients with RA in stable remission, and to clarify the role of Tfr cells in the pathogenesis of RA . Methods Levels of Tfr cells and follicular helper T (Tfh) cells in the peripheral blood of 39 patients with active RA , 39 patients with RA in stable remission, and 33 healthy controls were detected by flow cytometry. The function of Tfr cells was measured by coculturing them with Tfh cells and B cells. Activated CD 45 RA −FoxP3 high Tfr cells were also analyzed. Clinical indicators, including serum Ig and autoantibody levels, were tested, and correlations with Tfr cells were systematically analyzed. The Disease Activity Score in 28 joints ( DAS 28) was calculated, and correlation analysis with Tfr cells was conducted. Results The level of CD 4+ CXCR 5+FoxP3+ Tfr cells and the Tfr cell:Tfh cell ratio in peripheral blood from patients with RA in stable remission were significantly increased compared with the same measures in patients with active RA and in healthy controls. The function of Tfr cells was enhanced, and the activated CD 45 RA −FoxP3 high Tfr cell subset was increased in patients with RA in stable remission compared with healthy controls. Furthermore, the number of Tfr cells in RA patients was inversely correlated with IgG, rheumatoid factor, and anti–cyclic citrullinated peptide as well as with the DAS 28. Conclusion Circulating Tfr cells are increased as patients with RA achieve stable remission of disease, and increased Tfr cells can suppress autoimmunity in RA patients to stabilize their condition. Our results provide novel insight into RA pathogenesis.