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Lubricin Restoration in a Mouse Model of Congenital Deficiency
Author(s) -
Hill Adele,
Waller Kimberly A.,
Cui Yajun,
Allen Justin M.,
Smits Patrick,
Zhang Ling X.,
Ayturk Ugur M.,
Hann Steven,
Lessard Samantha G.,
Zurakowski David,
Warman Matthew L.,
Jay Gregory D.
Publication year - 2015
Publication title -
arthritis and rheumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.106
H-Index - 314
eISSN - 2326-5205
pISSN - 2326-5191
DOI - 10.1002/art.39276
Subject(s) - cartilage , medicine , boundary lubrication , ex vivo , gene expression , pathology , gene , in vivo , anatomy , biology , chemistry , genetics , lubricant , organic chemistry
Objective Congenital deficiency of the principal boundary lubricant in cartilage (i.e., lubricin, encoded by the gene PRG4 ) increases joint friction and causes progressive joint failure. This study was undertaken to determine whether restoring lubricin expression in a mouse model would prevent, delay, or reverse the disease process caused by congenital deficiency. Methods Using genetically engineered lubricin‐deficient mice, we restored gene function before conception or at ages 3 weeks, 2 months, or 6 months after birth. The effect of restoring gene function (i.e., expression of lubricin) on the tibiofemoral patellar joints of mice was evaluated histologically and by ex vivo biomechanical testing. Results Restoring gene function in mice prior to conception prevented joint disease. In 3‐week‐old mice, restoring gene function improved, but did not normalize, histologic features of the articular cartilage and whole‐joint friction. In addition, cyclic loading of the joints produced fewer activated caspase 3–containing chondrocytes when lubricin expression was restored, as compared to that in littermate mice whose gene function was not restored (nonrestored controls). Restoration of lubricin expression in 2‐month‐old or 6‐month‐old mice had no beneficial effect on histopathologic cartilage damage, extent of whole‐joint friction, or activation of caspase 3 when compared to nonrestored controls. Conclusion When boundary lubrication is congenitally deficient and cartilage becomes damaged, the window of opportunity for restoring lubrication and slowing disease progression is limited.