Premium
Brief Report: A Novel Application of Buccal Micronucleus Cytome Assay in Systemic Lupus Erythematosus: A Case–Control Study
Author(s) -
AlRawi Ziad S.,
Gorial Faiq I.,
Tawfiq Rana Fawzi,
Mohammed Ahmed Khaled,
AlNaaimi Ahmed S.,
Al'aadhmi Mohammed A.,
Hayyawi Ali A.
Publication year - 2014
Publication title -
arthritis and rheumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.106
H-Index - 314
eISSN - 2326-5205
pISSN - 2326-5191
DOI - 10.1002/art.38764
Subject(s) - micronucleus , buccal swab , micronucleus test , buccal administration , binucleated cells , systemic lupus erythematosus , immunology , biology , medicine , pathology , microbiology and biotechnology , bioinformatics , disease , toxicity
Objective Systemic lupus erythematous (SLE) is a systemic autoimmune disease, and both genetic and environmental factors are implicated in its pathogenesis. The aim of this study was to assess cellular and nuclear morphology of SLE using a buccal micronucleus cytome assay. Methods This study included 58 SLE patients and 58 healthy age‐ and sex‐matched controls. Patients' baseline characteristics were recorded, and a buccal micronucleus cytome assay was used to measure cellular and nuclear abnormalities in both groups. Results Significantly higher frequencies of micronuclei, nuclear buds, binucleated cells, basal cells, condensed chromatin cells, karyorrhectic cells, pyknotic cells, and karyolytic cells were seen in SLE patients as compared to controls. A buccal micronucleus cytome assay score of ≥4 had the highest accuracy (93.1%), almost perfect positive predictive value (98.1% at 50% pretest probability and 99.8% at 90% pretest probability), 87.9% sensitivity, and 98.3% specificity for the diagnosis of SLE. Conclusion Biomarkers of DNA damage, proliferative potential, and cell death were significantly increased in SLE patients. The buccal micronucleus cytome assay was a valid and easy way for clinicians to assess individuals at high risk of developing SLE.