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Reverse Interferon Signature Is Characteristic of Antigen‐Presenting Cells in Human and Rat Spondyloarthritis
Author(s) -
Fert Ingrid,
Cagnard Nicolas,
Glatigny Simon,
Letourneur Franck,
Jacques Sébastien,
Smith Judith A.,
Colbert Robert A.,
Taurog Joel D.,
Chiocchia Gilles,
Araujo Luiza M.,
Breban Maxime
Publication year - 2014
Publication title -
arthritis and rheumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.106
H-Index - 314
eISSN - 2326-5205
pISSN - 2326-5191
DOI - 10.1002/art.38318
Subject(s) - biology , transgene , genetically modified mouse , cxcl10 , interferon , signal transduction , cytokine , transcriptome , immunology , socs3 , chemokine , irf1 , microbiology and biotechnology , gene expression , gene , immune system , stat3 , genetics
Objective In HLA–B27–transgenic rats, the development of a disorder that mimics spondyloarthritis (SpA) is highly correlated with dendritic cell (DC) dysfunction. The present study was undertaken to analyze the underlying mechanisms of this via transcriptome analysis. Methods Transcriptome analysis of ex vivo–purified splenic CD103+CD4+ DCs from B27‐transgenic rats and control rats was performed. Transcriptional changes in selected genes were confirmed by quantitative reverse transcriptase–polymerase chain reaction. A meta‐analysis of our rat data and published data on gene expression in macrophages from ankylosing spondylitis (AS) patients was further performed. Results Interferon (IFN) signaling was the most significantly affected pathway in DCs from B27‐transgenic rats; the majority of genes connected to IFN were underexpressed in B27‐transgenic rats as compared to controls. This pattern was already present at disease onset, persisted over time, and was conserved in 2 disease‐prone B27‐transgenic rat lines. In DCs from B27‐transgenic rats, we further found an up‐regulation of suppressor of cytokine signaling 3 (which may account for reverse IFN signaling) and a down‐regulation of interleukin‐27 (a cytokine that opposes Th17 differentiation and promotes Treg cells). The meta‐analysis of data on conventional DCs from rats and data on monocyte‐derived macrophages from humans revealed 7 IFN‐regulated genes that were negatively regulated in both human and rat SpA (i.e., IRF1, STAT1, CXCL9, CXCL10, IFIT3, DDX60 , and EPSTI1) . Conclusion Our results suggest that expression of HLA–B27 leads to a defect in IFNγ signaling in antigen‐presenting cells in both B27‐transgenic rats and SpA patients, which may result in Th17 expansion and Treg cell alteration (as shown in B27‐transgenic rats) and contribute to disease pathogenesis.