Open AccessThe rheumatoid arthritis–associated allele HLA–DR10 ( DRB1*1001 ) shares part of its repertoire with HLA–DR1 ( DRB1*0101 ) and HLA–DR4 ( DRB*0401 )
Author(s) -
Alvarez Iñaki,
Collado Javier,
Daura Xavier,
Colomé Nuria,
RodríguezGarcía Marta,
Gallart Teresa,
Canals Francesc,
Jaraquemada Dolores
Publication year - 2008
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.23503
Subject(s) - allele , human leukocyte antigen , epitope , biology , rheumatoid arthritis , peptide , immunology , repertoire , peptide sequence , genetics , antigen , gene , biochemistry , physics , acoustics
Objective To identify the peptide anchor motif for the rheumatoid arthritis (RA)–related HLA allele, DR10, and find shared natural ligands or sequence similarities with the other disease‐associated alleles, DR1 and DR4. Methods The HLA–DR10–associated peptides were purified, and a proportion of these natural ligands were de novo sequenced by mass spectrometry. Based on crystallographic structures, the complexes formed by peptide influenza virus hemagglutinin HA306–318 with DR1, DR4, and DR10 were modeled, and binding scores were obtained. Results A total of 238 peptides were sequenced, and the anchor motif of the HLA–DR10 peptide repertoire was defined. A large proportion of the DR10‐associated peptides had the structural features to bind DR1 and DR4 but were theoretical nonbinders to the negatively associated alleles DR15 and DR7. Among the sequenced ligands, 10 had been reported as ligands to other RA‐associated alleles. Modeling data showed that peptide HA306–318 can bind DR1, DR4, and DR10 with similar affinities. Conclusion The data show the presence of common peptides in the repertoires of RA‐associated HLA alleles. The combination of the shared epitope present in DR1, DR4, and DR10 together with common putative arthritogenic peptide(s) could influence disease onset or outcome.