Open AccessPrevention of cartilage destruction with intraarticular osteoclastogenesis inhibitory factor/osteoprotegerin in a murine model of osteoarthritis
Author(s) -
Shimizu Sadanori,
Asou Yoshinori,
Itoh Soichiro,
Chung UngIl,
Kawaguchi Hiroshi,
Shinomiya Kenichi,
Muneta Takeshi
Publication year - 2007
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.22941
Subject(s) - osteoprotegerin , osteoarthritis , chondrocyte , medicine , cartilage , immunostaining , endocrinology , tunel assay , in vivo , apoptosis , endogeny , pathology , immunohistochemistry , chemistry , anatomy , receptor , biology , biochemistry , alternative medicine , microbiology and biotechnology , activator (genetics)
Objective To investigate the effect of osteoclastogenesis inhibitory factor/osteoprotegerin (OPG) on chondrocytes in the development of osteoarthritis (OA) in vivo. Methods To determine the role of endogenous OPG in the progression of OA, OA was surgically induced in OPG +/− mice and their wild‐type (WT) littermates. To determine the role of exogenous OPG, knee joints of C57BL/6J mice with surgically induced OA were injected intraarticularly with recombinant human OPG (rHuOPG) or vehicle 5 times a week. All mice were euthanized 4 weeks after OA induction; joints were harvested and evaluated immunohistochemically. Results Although OA changes were induced in both WT and OPG +/− mice, the degenerative changes in the articular cartilage were significantly enhanced in OPG +/− mice. In C57BL/6J mice with surgically induced OA, intraarticular OPG administration protected the articular cartilage from the progression of OA. The Mankin and cartilage destruction scores in OPG‐treated animals were ∼50% of those seen in the control group. Furthermore, OPG administration significantly protected articular cartilage thickness. Findings of the TUNEL assay indicated that rHuOPG prevented chondrocyte apoptosis in joints with surgically induced OA. Results of immunostaining indicated that OPG protein was detected in the synovium and in resident chondrocytes at higher levels in the OPG‐treated group than in the control group. Conclusion These data indicate that endogenous OPG had a protective effect against the cartilage destruction that occurs during OA progression. Furthermore, direct administration of rHuOPG to articular chondrocytes prevented cartilage destruction in an experimental murine model of OA via prevention of chondrocyte apoptosis.