Open AccessLIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis
Author(s) -
Edwards J. R.,
Sun S. G.,
Locklin R.,
Shipman C. M.,
Adamopoulos I. E.,
Athanasou N. A.,
Sabokbar A.
Publication year - 2006
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.21821
Subject(s) - rankl , osteoclast , osteoprotegerin , tumor necrosis factor alpha , receptor , bone resorption , chemistry , cytokine , endocrinology , rank ligand , medicine , immunology , peripheral blood mononuclear cell , cancer research , activator (genetics) , biochemistry , in vitro
Objective Human osteoclast formation from mononuclear phagocyte precursors involves interactions between tumor necrosis factor (TNF) ligand superfamily members and their receptors. LIGHT is a transmembrane protein expressed and shed from the surface of activated T cells. Since activated T cells have been implicated in osteoclastogenesis in rheumatoid arthritis (RA), this study sought to determine whether LIGHT can regulate RANKL/cytokine‐induced osteoclast formation, to identify the mechanism by which LIGHT influences osteoclastogenesis, and to investigate the presence of LIGHT in the serum of RA patients. Methods The effect of LIGHT on human and murine osteoclast formation was assessed in the presence and absence of neutralizing reagents to known osteoclastogenic factors. Serum levels of LIGHT in RA patients were measured by enzyme‐linked immunosorbent assay. Results In the presence and absence of RANKL, LIGHT induced osteoclast formation from both human peripheral blood mononuclear cells and murine macrophage precursors, in a dose‐dependent manner, whereas no inhibition was observed by adding osteoprotegerin, RANK:Fc, TNFα, or interleukin‐8 or by blocking the LIGHT receptors herpesvirus entry mediator or lymphotoxin β receptor. However, formation of osteoclasts was significantly decreased by the soluble decoy receptor for LIGHT, DcR3, and by blocking antibodies to the p75 component of the TNF receptor. A significant increase in LIGHT levels in the serum of RA patients compared with normal controls was also noted. Conclusion Our results indicate that LIGHT promotes RANKL‐mediated osteoclastogenesis and that it can induce osteoclast formation by a mechanism independent of RANKL. The increased concentration of LIGHT in patients with RA raises the possibility that LIGHT may play a role in immunopathogenic conditions that are associated with localized or systemic bone loss.