Open AccessDetection of anticentromere antibodies using recombinant human CENP‐A protein
Author(s) -
Sun Dongxu,
Martinez Antigona,
Sullivan Kevin F.,
Sharp Gordon C.,
Hoch Sallie O.
Publication year - 1996
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780390520
Subject(s) - recombinant dna , antibody , antigen , microbiology and biotechnology , biology , virology , chemistry , immunology , biochemistry , gene
Objective. To evaluate CENP‐A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP‐A). Methods. Human CENP‐A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full‐length recombinant polypeptide by immunoblot and by enzyme‐linked immunosorbent assay (ELISA). Results. Of the ACA+ sera studied (n = 38), 95% were positive when tested against the rCENP‐A in the ELISA system. Of the ACA‐ sera (n = 100), only 2% gave false‐positive results in the assay. There was good correlation between the recombinant and bona fide antigens in assaying for ACA reactivity. Conclusion. CENP‐A is a significant ACA target. The availability of the rCENP‐A assay is a valuable adjunct to the previously described rCENP‐B assay in analyses of the clinical significance of ACA.