Open AccessSensitivity and specificity of anti‐Jo‐1 antibodies in autoimmune diseases with myositis
Author(s) -
VázquezAbad Dolores,
Rothfield Naomi F.
Publication year - 1996
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780390218
Subject(s) - polymyositis , dermatomyositis , medicine , myositis , connective tissue disease , rheumatoid arthritis , mixed connective tissue disease , antibody , systemic disease , autoimmune disease , immunology , autoantibody , immunopathology , scleroderma (fungus) , pathology , inoculation
Objective . To determine the sensitivity and specificity of anti‐Jo‐1 in systemic sclerosis (SSc) patients with and without myositis. Methods . Immunoblots on HeLa nuclei were used to screen sera from 554 consecutive connective tissue disease patients. Those who had 45–55‐kd bands, all patients with polymyositis/dermatomyositis (PM/DM), and a random selection of SSc, Raynaud's disease, systemic lupus erythematosus, and rheumatoid arthritis patients were also studied by anti–Jo‐1 enzyme‐linked immunosorbent assay and by immunoblots on rabbit pooled aminoacyl‐transfer RNA synthetase. Results . Anti–Jo‐1 was present only in 8 of the 40 PM/DM patients. Conclusion . Anti–Jo‐1 is specific for PM/DM.