Open AccessDepletion of γ/δ T cells does not prevent or ameliorate, but rather aggravates, rat adjuvant arthritis
Author(s) -
Pelegrí Carme,
Kühnlein Petra,
Buchner Eberhard,
Schmidt Carsten B.,
Franch Angels,
Castell Margarida,
Hünig Thomas,
Emmrich Frank,
Kinne Raimund W.
Publication year - 1996
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780390206
Subject(s) - medicine , arthritis , adjuvant , immunology , t cell , rheumatoid arthritis , lymph , flow cytometry , pathology , immune system
Objective. To investigate the role of γ/δ T cells in Mycobacterium tuberculosis –induced rat adjuvant arthritis. Methods. Rats with adjuvant arthritis were injected with the anti–T cell receptor γ/δ (anti‐TCR γ/δ) monoclonal antibody V65 according to a preventive protocol, a pre–arthritis peak protocol, and a late therapeutic protocol. Arthritis severity and joint destruction were monitored, and depletion of target cells was analyzed by flow cytometry. Results. Although all protocols led to successful depletion of TCRγ/δ bright cells in peripheral blood and lymph nodes, none of the regimens influenced clinical parameters of adjuvant arthritis. If rats were treated before the clinical peak of adjuvant arthritis, however, joint destruction was significantly more severe than in vehicle‐treated rats. Conclusion. Rat adjuvant arthritis is not promoted or perpetuated by γ/δ T cells. Aggravation of joint destruction with pre–arthritis peak anti‐γ/δ treatment suggests a stage‐dependent protective role of γ/δ T cells in adjuvant arthritis.