Open AccessSpecificity of autoantibodies for recombinant 60–kd and 52–kd ro autoantigens
Author(s) -
Williams Clair E. ST.,
Burch James A.,
Saitta Michael
Publication year - 1994
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780370916
Subject(s) - recombinant dna , autoantibody , antigen , lupus erythematosus , immunoprecipitation , immunology , microbiology and biotechnology , autoimmune disease , antibody , medicine , connective tissue disease , chemistry , biology , biochemistry , gene
Objective . To compare patterns of Ro autoantigen recognition in primary Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE). Methods . Sera were tested by enzyme–linked immunosorbent assay (ELISA) and immunoprecipitation for reactivity with recombinant human 60–kd and 52–kd Ro proteins. Results . SLE and primary SS sera exhibited similar patterns of reactivity with the recombinant 52–kd protein. However, a greater proportion of sera from primary SS patients than from SLE patients did not react with recombinant 60–kd protein in ELISA yet precipitated its translation product in solution–phase assays. Conclusion . Disease state can influence recognition of the recombinant 60–kd Ro antigen.