Open AccessInhibition of Collagenase Activity by N ‐Chlorotaurine, a Product of Activated Neutrophils
Author(s) -
Davies Joanna M. S.,
Horwitz David A.,
Davies Kelvin J. A.
Publication year - 1994
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780370317
Subject(s) - collagenase , chemistry , arthritis , microbial collagenase , interstitial collagenase , matrix metalloproteinase , metalloproteinase , microbiology and biotechnology , biochemistry , immunology , enzyme , medicine , biology
Objective . To study the effects of N ‐chlorotaurine on collagenase activity, as a model of the effects of neutrophil activation in inflammatory arthritis. Methods . Collagen degradation by collagenase was measured by the release of acid‐soluble counts from 3 H‐collagen. Results . N ‐chlorotaurine inhibited the degradation of collagen by bacterial collagenase. This result is explained by a direct inhibition/inactivation of collagenase, since N ‐chlorotaurine had no effect on the proteolytic susceptibility of collagen itself. The effect appears to be specific to N ‐chlorotaurine since N ‐chloroalanine, N ‐chloroleucine, and HOCl/OCl ‐ failed to inhibit collagenase; in fact, N ‐chloroalanine and N ‐chloroleucine actually increased the proteolytic susceptibility of collagen. Conclusion . N ‐chlorotaurine may minimize damage to cartilaginous joint structures in inflammatory arthritis by inhibiting/inactivating collagenase.