Open AccessGeneration and molecular analyses of two rheumatoid synovial fluid–derived IgG rheumatoid factors
Author(s) -
Lu Edward W.,
Deftos Michael,
Olee Tsaiwei,
Huang DeFeng,
SotoGil Rafael W.,
Carson Dennis A.,
Chen Pojen P.
Publication year - 1993
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780360709
Subject(s) - hypervariable region , rheumatoid factor , synovial fluid , rheumatoid arthritis , immunology , gene , immunoglobulin g , antibody , gene duplication , microbiology and biotechnology , biology , medicine , chemistry , genetics , pathology , osteoarthritis , alternative medicine
Objective. To study the Ig genes that encode IgG rheumatoid factor (IgG‐RF) from rheumatoid synovial fluid. Methods. We used rheumatoid synovial fluid B cells to generate IgG‐RF–secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences. Results. Two monospecific IgG‐RFs were obtained. Sequence analysis of the RFs revealed a new Vλ gene family (designated Vλ9) and extensive somatic diversification, including a duplication‐insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region. Conclusion. The data provide further support for an antigen‐driven response in the sustained production of potentially pathogenic IgG‐RFs in rheumatoid synovium.