Open AccessT cell receptor vβ repertoire of double‐negative α/β t cells in patients with systemic sclerosis
Author(s) -
Sakamoto Akemi,
Sumida Takayuki,
Maeda Toshiro,
Itoh Michihiro,
Asai Takayoshi,
Takahashi Hidenori,
Yoshida Shouji,
Koike Takao,
Tomioka Hisao,
Yoshida Sho
Publication year - 1992
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780350815
Subject(s) - receptor , double negative , multiple sclerosis , medicine , immunology , chemistry , t cell , immune system
Objective. To analyze the T cell receptor V β gene on double‐negative (DN) α/β T cells, which are increased in number, on peripheral blood lymphocytes (PBL) from patients with systemic sclerosis (SSc). Methods. The DN α/β T cells were sorted by flow cytometry from PBL obtained from 3 patients with SSc. The V β repertoire was analyzed by polymerase chain reaction. Results. Only 1 or 2 V β genes (V β 5/7, 5, or 17) were predominantly expressed on DN α/β T cells from these 3 patients. Conclusion. The V β repertoire on DN α/β T cells in PBL from patients with SSc is rather restricted.