Open AccessIncreased production of endothelin‐1 in patients with inflammatory arthritides
Author(s) -
Miyasaka Nobuyuki,
Hirata Yukio,
Ando Kenji,
Sato Kazuto,
Morita Hideho,
Shichiri Masayoshi,
Kanno Kazuo,
Tomita Kimio,
Marumo Fumiaki
Publication year - 1992
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780350406
Subject(s) - radioimmunoassay , synovial fluid , in vivo , autocrine signalling , medicine , paracrine signalling , endocrinology , endothelin receptor , endothelin 1 , in vitro , immunology , receptor , chemistry , pathology , biology , biochemistry , alternative medicine , microbiology and biotechnology , osteoarthritis
Objective. Endothelin‐1 (ET‐1) is a potent vasoconstrictive peptide, but its precise mechanism of action in vivo has remained unknown. Methods. We measured ET‐1 activity by radioimmunoassay, in both plasma and synovial fluid from patients with inflammatory arthritides. Results. ET‐1–like immunoreactivity was found in synovial fluid, at levels severalfold higher than those in plasma. Reverse‐phase high‐performance liquid chromatography showed an elution profile corresponding to actual ET‐1. A single class of high‐affinity binding sites for ET‐1 in cultured synovial cells was also detected. Furthermore, ET‐1 induced mild DNA synthesis in synovial cells. Conclusion. Taken together, these results indicate that ET‐1 might contribute to the synovial proliferation seen in inflammatory arthritides, in an autocrine/paracrine manner.