Open AccessGeneration and Characterization of two Monoclonal Self‐Associating IgG Rheumatoid Factors from a Rheumatoid Synovium
Author(s) -
Lu Edward W.,
Deftos Michael,
Tighe Helen,
Carson Dennis A.,
Chen Pojen P.
Publication year - 1992
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780350115
Subject(s) - rheumatoid factor , rheumatoid arthritis , immune system , immunology , immunoglobulin g , medicine , monoclonal antibody , antibody , immune complex , synovial membrane
Objective. To study IgG rheumatoid factor (RF) from rheumatoid synovium. Methods. We fused the K6H6/B5 human—mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG‐RF—secreting hybridomas. Results. The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self‐associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG‐RFs in rheumatoid synovium. Conclusion. IgG‐RF—secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG‐RF.