Open AccessCrystal‐induced neutrophil activation. I. Initiation and modulation of calcium mobilization and superoxide production by microcrystals
Author(s) -
Naccache Paul H.,
Grimard Marléne,
Roberge Charles J.,
Gilbert Caroline,
Poubelle Patrice E.,
Lussier André,
De Médicis Rinaldo
Publication year - 1991
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780340311
Subject(s) - respiratory burst , calcium , superoxide , chemistry , cytochalasin b , colchicine , pertussis toxin , protein kinase c , granulocyte , chemotaxis , endocrinology , kinase , medicine , biochemistry , biology , signal transduction , enzyme , g protein , receptor , in vitro
The effects of monosodium urate and calcium pyrophosphate dihydrate crystals on the levels of cytoplasmic free calcium and on the oxidative burst in normal human blood neutrophils were examined. The pattern of sensitivity to granulocyte–macrophage colony‐stimulating factor, colchicine, cytochalasin B, pertussis toxin, diglyceride kinase, and protein kinase C inhibitors differentiated the mechanism(s) of neutrophil activation by the crystals from that involved in the responses to soluble chemotactic factors and indicated that individual crystals can use several activation pathways.