Open AccessGlycoprotein specificity of cold‐reactive IgM antilymphocyte autoantibodies in systemic lupus erythematosus
Author(s) -
Mimura Toshihide,
Fernsten Philip,
Shaw Melody,
Jarjour Wael,
Winfield John B.
Publication year - 1990
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780330824
Subject(s) - autoantibody , antibody , immunology , lupus erythematosus , cold agglutinin , glycoprotein , medicine , microbiology and biotechnology , chemistry , biology
Sera from patients with systemic lupus erythematosus frequently contain IgM antibodies to glycoproteins of M r 46,000 and ∼200,000 isolated from nonionic detergent lysates of mature T cells by affinity chromatography with solid‐phase wheat germ agglutinin. Autoantibodies of this specificity correlate strongly with the presence of IgM anti‐T cell autoantibodies, as determined by independent indirect immunofluorescence and complement‐dependent microcytotoxicity assays, and are specifically absorbed by incubation of patient serum with viable T cells. Collectively, the data suggest that gp46 and, to a lesser extent, gp∼200 represent major targets of IgM antilymphocyte autoantibodies in systemic lupus erythematosus.