Open AccessImmunologic dysfunction in scleroderma: evidence for increased mast cell releasability and hla–dr positivity in the dermis
Author(s) -
Pearson Mark E.,
Clark Huff J.,
Giorno Ralph C.,
Panicheewa Sucheelar,
Claman Henry N.,
Steigerwald James C.
Publication year - 1988
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780310514
Subject(s) - dermis , immunoperoxidase , mast cell , medicine , scleroderma (fungus) , histamine , systemic scleroderma , pathology , pathogenesis , staining , immunology , skin biopsy , biopsy , antibody , monoclonal antibody , disease , inoculation
To investigate the role of mast cells and cell‐mediated immunity in the pathogenesis of scleroderma, we studied wheal size after skin testing with compound 48/80, a liberator of mast cell histamine, and demonstrated increased mast cell releasability in skin that appeared normal, adjacent to involved skin. Immunofluorescent staining for HLA–DR showed dermal positivity in 12 of 13 involved‐ and 9 of 13 uninvolved‐skin biopsy specimens from scleroderma patients, compared with only 1 of 10 controls. By immunoperoxidase staining, most of the DR positivity was found in fibroblast‐like cells. These findings further support the notion of immunologic dysfunction in scleroderma.