Open AccessQuantitative immunoassay of anti‐la antibodies using purified recombinant la antigen
Author(s) -
T. Clair E. William S,
Pisetsky David S.,
Reich Charles F.,
Chambers Jasemine C.,
Keene Jack D.
Publication year - 1988
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780310407
Subject(s) - recombinant dna , microbiology and biotechnology , antibody , immunoassay , chemistry , precipitin , fusion protein , antigen , enzyme , biology , biochemistry , immunology , gene
A purified recombinant La fusion protein was tested in an enzyme‐linked immunosorbent assay to quantitate anti‐La responses. This protein contained the immunodominant region of the La molecule fused to β‐galactosidase. In solid‐phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant‐based enzyme‐linked immunosorbent assay proved to be a sensitive method for the detection of anti‐La binding, and it accurately distinguished anti‐La precipitin positive sera from normal sera.