Open AccessInterleukin‐1 activates phospholipase a 2 IN HUMAN SYNOVIAL CELLS
Author(s) -
Gilman Steven C.,
Chang Joseph,
Zeigler Pamela R.,
Uhl Joanne,
Mochan Eugene
Publication year - 1988
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780310118
Subject(s) - synovial fluid , rheumatoid arthritis , secretion , phospholipase a2 , endocrinology , interleukin , osteoarthritis , medicine , chemistry , prostaglandin e2 , plasminogen activator , phospholipase a , immunology , cytokine , pathology , biochemistry , enzyme , alternative medicine
Interleukin‐1 (IL‐1) treatment of synovial cells from rheumatoid arthritis and osteoarthritis patients resulted in a dose‐dependent secretion of phospholipase A 2 (PLA 2 ). IL‐1 also stimulated prostaglandin E 2 and plasminogen activator synthesis, in parallel with PLA 2 activation; all 3 were detectable within 6 hours of IL‐1 treatment and peaked by 24 hours. Synovial cell PLA 2 required calcium (5 m M ) and a neutral pH (7.5) for maximal activity and appears similar to the PLA 2 in synovial fluid, which has been described previously. We conclude that PLA 2 can be induced by IL‐1, and its secretion may contribute significantly to the inflammatory actions of IL‐1.