Open AccessDetection of anticentromere antibodies using cloned autoantigen cenp‐Bl
Author(s) -
Rothfield N.,
Whitaker D.,
Bordwell B.,
Weiner E.,
Senecal J.L.,
Earnshaw W.
Publication year - 1987
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780301214
Subject(s) - epitope , fusion protein , immunofluorescence , antibody , connective tissue disease , scleroderma (fungus) , antigen , microbiology and biotechnology , mixed connective tissue disease , autoantibody , indirect immunofluorescence , autoimmune disease , medicine , immunology , biology , recombinant dna , biochemistry , gene , inoculation
A solid‐phase enzyme‐linked immunosorbent assay has been established using a cloned fusion protein, C term CENP‐B [β‐gal], as antigen. The fusion protein carries the major epitope of CENP‐B, the major centromeric autoantigen. The enzyme‐linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud'S disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.