Open AccessCHARACTERIZATION OF RABBIT GENES FOR SYNOVIAL CELL COLLAGENASE
Author(s) -
Fini M. Elizabeth,
Gross Robert H.,
Brinckerhoff Constance E.
Publication year - 1986
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780291102
Subject(s) - collagenase , gene , microbiology and biotechnology , biology , synovial membrane , gene expression , microbial collagenase , cell , in situ hybridization , genome , rheumatoid arthritis , genetics , immunology , biochemistry , enzyme
To provide tools for understanding collagenase gene expression in rheumatoid arthritis, we have isolated and characterized genomic clones for rabbit synovial cell collagenase. These clones represent 2 types of collagenase gene, at least 1 of which is transcribed in synovial fibroblasts. By examining the rabbit genome in situ, we provide evidence that there are only 2 different synovial cell collagenase genes found in a haploid genome. Amplification of these genes is not a mechanism for collagenase messenger RNA induction by phorbol esters.