Open AccessSynthesis of electrophoretically restricted IgG by cultured rheumatoid synovium
Author(s) -
Hoffman Wayne L.,
Douglass Rita R.,
Donald Smiley J.
Publication year - 1984
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780270903
Subject(s) - isoelectric focusing , isoelectric point , chromatofocusing , rheumatoid arthritis , microbiology and biotechnology , immunoglobulin g , immunology , chemistry , medicine , pathology , antibody , biology , biochemistry , enzyme
IgG synthesized by excised rheumatoid (RA) synovia was separated according to isoelectric point using chromatofocusing and isoelectric focusing. Over 30% of the IgG synthesized by 9 of 11 synovia had an isoelectric point >8 (cathodal shift), while 55% of the synovia secreted IgG showing oligoclonal banding by isoelectric focusing. Oligoclonal serum IgG was seen in 17% of RA patients and in only 3% of hospitalized patients without RA. These results emphasize the selective character of the antigenic stimulus of the RA synovial lymphoid infiltrate.