Open AccessHyaluronate Synthesis by Synovial Villi in Organ Culture
Author(s) -
Myers Stephen L.,
Christine Theresa A.
Publication year - 1983
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780260610
Subject(s) - glycosaminoglycan , organ culture , hyaluronic acid , glucosamine , cell culture , synovial membrane , in vitro , chemistry , pathology , synovial fluid , biology , anatomy , immunology , medicine , biochemistry , osteoarthritis , arthritis , genetics , alternative medicine
Individual canine synovial villi were used to establish short‐term synovial organ cultures. These villi incorporated 3 H‐glucosamine into highly‐polymerized 3 H‐hyaluronic acid ( 3 H‐HA), which was the only 3 H‐glycosaminoglycan identified in the culture medium. Some 3 H‐HA, and larger amounts of other 3 H‐glycosaminoglycans, were recovered from cultured tissues. Culture medium 3 H‐HA content was proportional to the surface area of cultured villi. Organ cultures of nonvillous synovium were compared with villi; nonvillous cultures synthesized less 3 H‐HA per mm 2 of their synovial intimal surface than villi. These cultures complement cell culture techniques for in vitro studies of synovial lining cell function.