Open AccessCytotoxic activity of sera from scleroderma and other connective tissue diseases. lack of cellular and disease specificity
Author(s) -
Shanahan William R.,
Korn Joseph H.
Publication year - 1982
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780251201
Subject(s) - connective tissue disease , medicine , scleroderma (fungus) , connective tissue , fibroblast , rheumatoid arthritis , endothelial stem cell , mixed connective tissue disease , immunology , cytotoxic t cell , pathology , autoimmune disease , biology , cell culture , antibody , in vitro , biochemistry , genetics , inoculation
It has been suggested that serum‐mediated endothelial cell injury in scleroderma might contribute to disease pathogenesis. We compared the effect of serum from 28 scleroderma patients on human umbilical cord vein endothelial cell and human foreskin fibroblast proliferation with sera from 28 healthy controls, 13 patients with isolated Raynaud's disease, 22 patients with systemic lupus erythematosus, 19 patients with rheumatoid arthritis, and 15 patients with other connective tissue diseases. Five sera (2 scleroderma, 1 morphea, 1 rheumatoid arthritis, 1 systemic lupus erythematosus) markedly suppressed 3 H‐thymidine incorporation into both endothelial cells and fibroblasts (to > 3 SD below the mean of the control group). These sera were also cytotoxic to endothelial cells and fibroblasts in a 51 Cr release assay. Three additional sera (1 Raynaud's, 2 controls) suppressed endothelial cell proliferation moderately (>2 SD but <3 SD from control mean) but did not affect fibroblasts. Mean 3 H‐thymidine incorporation by endothelial cells and fibroblasts in scleroderma serum was comparable to that of the other disease and control groups. In contrast to previous studies, we found serum‐mediated endothelial cell cytotoxicity occurred infrequently in scleroderma, occurred also in other connective tissue diseases, and was without target cell specificity. Furthermore, scleroderma serum did not appear to stimulate fibroblast proliferation.