Open AccessMolecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluation
Author(s) -
Matter L.,
Schopfer K.,
Wilhelm J. A.,
Nyffenegger T.,
Parisot R. F.,
De Robertis E. M.
Publication year - 1982
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780251102
Subject(s) - anti nuclear antibody , ribonucleoprotein , antibody , antigen , immunology , medicine , computational biology , chemistry , biology , autoantibody , biochemistry , rna , gene
The specificity of antinuclear antibodies in sera from 54 patients with various rheumatic diseases was analyzed by immunoprecipitation of 32 P‐ or 35 S‐methionine‐labeled HeLa cell extracts. Of 35 sera giving a speckled and/or homogeneous immunofluorescence pattern in rat liver nuclei, 20 contained antibodies to nuclear ribonucleoproteins, which are defined by their ribonucleic acid and protein components. Four sera reacted with a nuclear antigen (Ga) which has not been described so far. Of 19 sera with antinucleolar antibodies, 18 did not react with nuclear or nucleolar ribonucleoproteins. Correlation of these molecularly defined antinuclear antibody specificities with immunofluorescence patterns and rheumatic diseases is discussed.