Open AccessIn vitro cartilage degradation by escherichia coli and staphylococcus aureus
Author(s) -
Smith R. Lane,
Merchant Thomas C.,
Schurman David J.
Publication year - 1982
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780250413
Subject(s) - staphylococcus aureus , cartilage , glycosaminoglycan , microbiology and biotechnology , chondrocyte , escherichia coli , chemistry , in vitro , viability assay , bacteria , biology , biochemistry , anatomy , genetics , gene
Effects of Escherichia coli and Staphylococcus aureus on cartilage and chondrocytes in culture are reported. Under these conditions, bacterial effects on cartilage degradation and cell viability are measured in the absence of inflammation. E coli causes a 28% loss and S aureus an 83% loss of cartilage glycosaminoglycan within 48 hours. Collagen content is unchanged. Both bacterial species induce chondrocyte death in explants and in monolayers within 48 hours. Bacterial effects on glycosaminoglycans and cell viability do not result from depletion of nutrients from the culture medium. Serum in the culture media inhibits the bacterial effects on cartilage degradation but does not prevent cell death.