Open AccessIn vitro hexosamine depletion of intact articular cartilage by e‐prostaglandins
Author(s) -
Fulkerson John P.,
LadenbauerBellis IngeMaria,
Chrisman O. Donald
Publication year - 1979
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780221011
Subject(s) - cartilage , catabolism , chemistry , in vitro , prostaglandin , proteases , prostaglandin e , articular cartilage , prostaglandin e2 , endocrinology , matrix (chemical analysis) , osteoarthritis , medicine , biochemistry , anatomy , metabolism , biology , pathology , enzyme , alternative medicine , chromatography
Short‐term incubations of intact canine articular cartilage slices with prostaglandins E 1 and E 2 caused significant losses of hexosamine from cartilage matrix compared to controls. Chloroquine, an inhibitor of DNA primer, prevented this prostaglandin‐induced hexosamine depletion. These data suggest that E‐prostaglandins may degrade articular cartilage directly through DNA‐dependent RNA synthesis of cathepsinlike proteases. Catabolism of articular cartilage probably involves degradation of existing matrix in addition to inhibition of synthesis.