Open AccessSearch for type‐c oncornavirus–related genetic information in tissues from patients with systemic lupus erythematosus
Author(s) -
Aulakh Gurmit S.,
Hicks John T.,
John Martin W.,
Phillips Paul E.
Publication year - 1978
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780210802
Subject(s) - virology , dna , virus , biology , genome , rna , nucleic acid thermodynamics , reverse transcriptase , gene , genetics
Single‐stranded 3 H‐DNA probes complementary to the RNA of Rauscher murine leukemia virus and of simian sarcoma virus were prepared using techniques that permitted complete transcription of the viral genome of each virus. These probes were used in DNA‐DNA hybridization studies with the cellular DNA from uncultured specimens of spleens and placentas of patients with systemic lupus erythematosus (SLE). No proviral DNA sequences related to these viruses were detected in these tissues. The results presented here do not support previously reported antigenic data implicating type‐C oncornavirus infection of these organs in SLE.